ZC3H4 Knockout cell line (Hep G2)

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ZC3H4 Knockout cell line (Hep G2) Order

ZC3H4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1848
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ZC3H4 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ZC3H4 Knockout cell line (Hep G2)
Species
Human
Gene Name
ZC3H4
Gene ID
23211
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript ZC3H4-201)
Validation: PCR+Sanger Sequencing
ZC3H4 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ZC3H4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ZC3H4 gene knockout ZC3H4 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ZC3H4 Knockout cell line (Hep G2)
ZC3H4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1848
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ZC3H4 Knockout cell line (HeLa)

    ZC3H4 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95882
    YKO-XN95882(HeLa)
  • ZC3H4 Knockout cell line (HCT 116)

    ZC3H4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95881
    YKO-XN95881(HCT 116)
  • ZC3H4 Knockout cell line (A549)

    ZC3H4 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95880
    YKO-XN95880(A549)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ZC3H4 Knockout cell line (HeLa)

    ZC3H4 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95882
      inquiry   More details
  • ZC3H4 Knockout cell line (HCT 116)

    ZC3H4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95881
      inquiry   More details
  • ZC3H4 Knockout cell line (A549)

    ZC3H4 Knockout cell line (A549)

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95880
      inquiry   More details

ZC3H4 Knockout cell line (Hep G2) Order

ZC3H4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1848
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ZC3H4 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ZC3H4 Knockout cell line (Hep G2)
Species
Human
Gene Name
ZC3H4
Gene ID
23211
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript ZC3H4-201)
Validation: PCR+Sanger Sequencing
ZC3H4 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ZC3H4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ZC3H4 gene knockout ZC3H4 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ZC3H4 Knockout cell line (Hep G2)
ZC3H4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1848
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ZC3H4 Knockout cell line (HeLa)

    ZC3H4 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95882
    YKO-XN95882(HeLa)
  • ZC3H4 Knockout cell line (HCT 116)

    ZC3H4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95881
    YKO-XN95881(HCT 116)
  • ZC3H4 Knockout cell line (A549)

    ZC3H4 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95880
    YKO-XN95880(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ZC3H4 Knockout cell line (HeLa)

    ZC3H4 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95882
      inquiry   More details
  • ZC3H4 Knockout cell line (HCT 116)

    ZC3H4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95881
      inquiry   More details
  • ZC3H4 Knockout cell line (A549)

    ZC3H4 Knockout cell line (A549)

    Organism: Human
    Gene Name: ZC3H4
    Gene ID: 23211
    Size: 1*10^6
    Catalog#: YKO-XN95880
      inquiry   More details

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